A Micro-Assayfor EstrogenReceptorin BreastTumorwith Use of 125I4beIe’J Estradiol
نویسندگان
چکیده
The concentration of estrogen receptor protein in breast-tumortissue is generallyexpressed in units of femtomoles of estradiol bound by the receptor per milligram of cytosol protein. The sensitivity of the estrogen receptor radioligand assay is therefore related to the specific activity of the steroid label used for the binding assay, the amount of the receptor protein in the volume of cytosolused, and the proteinconcentrationinthe cytosol.Inthis paper, we discuss factors affecting the sensitivity of the estrogen receptor assay and present various approaches for optimizing the assay. We also describe a procedure that involves a radioiodinated estradiol of high specificactivity, a micro-techniqueforpreparingtumor cytosol,and a micro-assay procedure withwhich the estrogen receptor protein can be measured in as little as 50 mg (wet weight) of tissue.
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